RNA

Part:BBa_K199014

Designed by: William Vernon   Group: iGEM09_MoWestern_Davidson   (2009-07-02)

AGGAC tRNA Suppressor (Produces Serine)

A serine tRNA was modified to have the anticodon loop 5' CUGUCCUAA 3'so that the anticodon GUCCU could be used to decode a frameshift mutation of AGGAC in a mRNA. Update: these tRNA parts do not work as expected, but instead appear to read four-base codons. See [http://www.ibc7.org/article/journal_v.php?sid=287]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 38
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aAGGAC tRNA Suppressor (Produces Serine)